KINARI-Mutagen Case Study: 1CRN Crambin

Figure 1: Protein 1CRN, Crambin, is a 46 amino acid protein composed of two alpha helices and a short anti-parallel beta sheet (1(a)). When rigidity analysis is performed on the non-mutated form of Crambin, the majority of the atoms in the proteins core are part of a single large rigid cluster (shown in purple))(1(b)). KINARI-Mutagen was used to generate several in-silico mutants of crambin. When excision was performed on residue 3, the size of the largest rigid cluster decreased(1(c)).